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水平凝胶电泳用于增强蛋白质 - RNA复合物的检测

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

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水平凝胶电泳用于增强蛋白质 - RNA复合物的检测

DOI:

10.3791/56031-v

•

06:36 min

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July 28, 2017

•

Megan E. Dowdle, Susanne Blaser Imboden, Sookhee Park, Sean P. Ryder, Michael D. Sheets

¹Department of Biomolecular Chemistry,University of Wisconsin School of Medicine and Public Health, ²Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School

Capítulos

00:05Título
00:35Preparing the Horizontal Native Polyacrylamide Gel
02:27Binding Reaction
03:23Analysis of Samples
04:12Imaging the Gel
05:04Results: Horizontal Gel Electrophoresis Enhances the Detection of Distinct RNA-protein Complexes
06:08Conclusion

Summary

Tadução automática

天然聚丙烯酰胺凝胶电泳是分析RNA蛋白相互作用的基础工具。传统上大多数实验都使用垂直凝胶。然而，水平凝胶提供了几个优点，例如在电泳期间监测复合物的机会。我们提供生成和使用水平天然凝胶电泳的详细协议。

Tags

Keywords: Horizontal Gel Electrophoresis Protein-RNA Complexes RNA Biochemistry RNA-protein Interactions Native Gel Electrophoresis Gel Box TBE Running Buffer Acrylamide Gel Binding Reaction Fluorescently Labeled RNA Bicaudal-c Protein

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