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延期共聚焦显像移植神经元在器官性切片培养胚胎小鼠脑使用<em>在Utero</em>电穿孔
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Neurociência
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JoVE Journal Neurociência
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
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延期共聚焦显像移植神经元在器官性切片培养胚胎小鼠脑使用<em>在Utero</em>电穿孔

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13:33 min

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July 25, 2017

DOI:

10.3791/55886-v

DOI:
10.3791/55886-v

•

13:33 min
July 25, 2017

•

1 Views
, , ,
1Institute of Molecular and Cellular Anatomy,Ulm University

Tags

Time-lapse Confocal ImagingMigrating NeuronsOrganotypic Slice CultureEmbryonic Mouse BrainIn Utero ElectroporationNeocortex DevelopmentNeuron PolarizationRadial MigrationMigration SpeedMigratory DirectionPregnant MouseLateral VentricleMicroinjectionDNA SolutionTweezers-type Electrodes

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Wiegreffe, C., Feldmann, S., Gaessler, S., Britsch, S. Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation. J. Vis. Exp. (125), e55886, doi:10.3791/55886 (2017).

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