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JoVE Journal Neuroscience
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
 

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Article DOI: 10.3791/55886-v • 13:33 min • July 25th, 2017
July 25th, 2017 •
1, 1, 1, 1
1Institute of Molecular and Cellular Anatomy, Ulm University

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This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.

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Time-lapse Confocal Imaging Migrating Neurons Organotypic Slice Culture Embryonic Mouse Brain In Utero Electroporation Neocortex Development Neuron Polarization Radial Migration Dynamic Properties Speed Profiles Average Migration Speed Migratory Direction Pregnant Mouse Warming Plate Petroleum Jelly Surgical Tape Sterile Gauze Abdominal Incision
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