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Biologia
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Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
JoVE Journal
Biologia
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JoVE Journal Biologia
Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

DOI:
10.3791/55844-v

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09:48 min

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June 30, 2017

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, ,
1Lawrence J. Ellison Institute for Transformative Medicine,University of Southern California (USC)

Capítulos

  • 00:05Título
  • 00:45Cell Plating
  • 02:12Drug Dosing and Image Acquisition
  • 04:39Image Analysis
  • 07:20Results: Fluorescence and Morphology Classification of Heterocellular Populations
  • 08:58Conclusion

Summary

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Tadução automática

We have developed a novel protocol for studying heterocellular population dynamics in response to perturbations. This manuscript describes an imaging-based platform that produces quantitative datasets for simultaneous characterization of multiple cellular phenotypes of heterocellular populations in a robust manner.

Tags

Heterocellular PopulationsQuantitative Imaging TechniquesCell DiscriminationSubpopulation AnalysisCell ResponseH3255 Tumor CellsCCD19LU FibroblastsErlotinibCell CountingCell SeedingDrug TreatmentFluorescence StainingMorphology-based Classification

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