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Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis
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1School of Life Science and Technology,ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University
Capítulos
- 00:05Título
- 00:59Preparation of Dissection Instruments and Reagents
- 02:10Isolation of Epithelial Cells from Rat Cauda Epididymides
- 06:09Patch-clamp Experiment
- 08:32Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
- 10:03Conclusion
Vi præsenterer en protokol, der kombinerer cellisolering og fuldcelle patch-clamp-optagelse til måling af de primære dissocierede epitelcellers elektriske egenskaber fra rotte-cauda-epididymiderne. Denne protokol tillader undersøgelse af de primære epididymepitelcellers funktionelle egenskaber for yderligere at belyse epididymis fysiologiske rolle.
