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Rewiring Neuronal Circuits: Un Nuevo Método Para La Extensión Neurítica Rápida Y La Conexión Neuronal Funcional
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Neurociência
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JoVE Journal Neurociência
Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection
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Rewiring Neuronal Circuits: Un Nuevo Método Para La Extensión Neurítica Rápida Y La Conexión Neuronal Funcional

8,638 Views

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10:26 min

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June 13, 2017

DOI:

10.3791/55697-v

DOI:
10.3791/55697-v

•

10:26 min
June 13, 2017

•

5 Views
, , , , ,
1Department of Physics,McGill University, 2Department of Neurology and Neurosurgery,Montreal Neurological Institute, 3Ananda Devices

Summary

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Este procedimiento describe cómo iniciar, extender y conectar rápidamente neuritas organizadas en cámaras microfluídicas usando perlas revestidas con poli-D-lisina fijadas a micropipetas que guían la elongación de las neuritas.

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Cite this Article

Magdesian, M. H., Anthonisen, M., Lopez-Ayon, G. M., Chua, X. Y., Rigby, M., Grütter, P. Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection. J. Vis. Exp. (124), e55697, doi:10.3791/55697 (2017).

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