Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

Margaret H. Magdesian; Madeleine Anthonisen; G. Monserratt Lopez-Ayon; Xue Ying Chua; Matthew Rigby; Peter Gr&#252;tter

doi:10.3791/55697

Journal

/

Neurosciences

/

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

Journal JoVE
Neurosciences

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Neurosciences

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

8,638 Views

•

10:26 min

•

June 13, 2017

DOI:

10.3791/55697-v

DOI:

10.3791/55697-v

•

10:26 min

June 13, 2017

•

5 Views

Margaret H. Magdesian^1,2,3, Madeleine Anthonisen¹, G. Monserratt Lopez-Ayon¹, Xue Ying Chua¹, Matthew Rigby¹, Peter Grütter¹

¹Department of Physics,McGill University, ²Department of Neurology and Neurosurgery,Montreal Neurological Institute, ³Ananda Devices

Summary

This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.

Explore More Videos

Neurite Extension Functional Neuronal Connection Neurite Growth Rate Synapse Formation Neural Network Single-cell Manipulation Experimental Reproducibility PDL Coating Microfluidic Devices Cell Culture Neurosciences

Read Article

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

Summary

Automatically generated

Explore More Videos