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光片为主的生活的荧光显微镜或固定和染色<em>赤拟谷盗</em>胚胎

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

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光片为主的生活的荧光显微镜或固定和染色<em>赤拟谷盗</em>胚胎

DOI:

10.3791/55629-v

•

10:15 min

•

April 28, 2017

•

Frederic Strobl^2,3, Selina Klees^2,3, Ernst H. K. Stelzer^2,3

¹Physical Biology,Buchmann Institute for Molecular Life Sciences (BMLS), ²Cluster of Excellence Frankfurt,Macromolecular Complexes, ³Goethe-Universität Frankfurt am Main – Campus Riedberg

Capítulos

00:05Título
00:47Embryo Mounting and Insertion into Sample Chamber
04:48Light Sheet-based Fluorescence Microscopy
06:08Embryo Retrieval Quality Control and Image Data Processing
08:05Results: Representative Embryo Imaging Analyses
09:42Conclusion

Summary

Tadução automática

成像昆虫胚胎与基于光的片材的荧光显微镜的形态已成为现有技术的状态。该协议概述并比较适合赤拟谷盗胚胎三个安装技术中，引入了两个新的定制的转基因系非常适合于实时成像，讨论必需的质量控制和指示当前实验的限制。

Tags

Light Sheet-based Fluorescence Microscopy Tribolium Castaneum Embryo Development Noninvasive Observation Cell Migration Cell Proliferation Agarose Column Agarose Hemisphere Sample Chamber Photobleaching Phototoxicity

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