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激光局部补骨脂素加合物的单分子分析
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Genética
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JoVE Journal Genética
Single Molecule Analysis of Laser Localized Psoralen Adducts
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激光局部补骨脂素加合物的单分子分析

6,510 Views

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11:46 min

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April 20, 2017

DOI:

10.3791/55541-v

DOI:
10.3791/55541-v

•

11:46 min
April 20, 2017

•

1 Views
, , , , , ,
1Laboratory of Molecular Gerontology,National Institute on Aging, National Institutes of Health

Summary

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激光器在对DNA损伤的细胞应答的研究经常使用的。然而，它们产生的病变，其间隔，频率，和碰撞与复制叉很少表征。在这里，我们描述了一种方法，使这些参数与激光局部间交联的决心。

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Cite this Article

Huang, J., Gali, H., Gichimu, J., Bellani, M. A., Pokharel, D., Paramasivam, M., Seidman, M. M. Single Molecule Analysis of Laser Localized Psoralen Adducts. J. Vis. Exp. (122), e55541, doi:10.3791/55541 (2017).

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