Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Single Molecule Analysis of Laser Localized Psoralen Adducts
6,518 Views
•
11:46 min
•
April 20, 2017
DOI:
Summary
Automatically generated
Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.
Read Article
Cite this Article
Huang, J., Gali, H., Gichimu, J., Bellani, M. A., Pokharel, D., Paramasivam, M., Seidman, M. M. Single Molecule Analysis of Laser Localized Psoralen Adducts. J. Vis. Exp. (122), e55541, doi:10.3791/55541 (2017).
Copy