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Single Molecule Analysis of Laser Localized Psoralen Adducts
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JoVE Journal Genetik
Single Molecule Analysis of Laser Localized Psoralen Adducts

Single Molecule Analysis of Laser Localized Psoralen Adducts

DOI:
10.3791/55541-v

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11:46 min

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April 20, 2017

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, , , , , ,
1Laboratory of Molecular Gerontology,National Institute on Aging, National Institutes of Health

Kapitel

  • 00:05Titel
  • 00:53Calibrating the Targeting Laser
  • 04:54Laser Localized Dig-TMP ICLs
  • 07:44Harvesting Cells and Stretching DNA Fibers
  • 09:51Results: Laser Localized Psoralen Adducts Reveal the DNA Damage Response to ICLs
  • 11:21Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.

Tags

Single Molecule AnalysisLaser Localized Psoralen AdductsDNA Interstrand CrosslinksDNA RepairLaser Activated CompoundMirror Slide CalibrationLaser TargetingBiological CalibrationTrimethylpsoralenGFP Tagged Repair FactorU2OS Cell Line

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