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体外体内の T, B, 骨髄細胞の抑制活性と移植から体液性応答の評価
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
DOI:

18:48 min

August 12, 2017

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Capítulos

  • 00:00Título
  • 00:37LEW.1W to LEW.1A Allograft Procedure
  • 02:18Isolation of Allograft APCs (pDCs)
  • 06:12Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
  • 10:30CFSE Labeling of Responder Cells to Measure their Proliferation
  • 12:09FACS Staining and Analysis After 6 Days of Coculture
  • 13:49In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
  • 16:33Representative Results
  • 18:03Conclusion

Summary

Tadução automática

ここでは、受信者からの明瞭な細胞のサブセットの抑制能力と受信者ドナーまたは外因性の抗原への免疫の状態、移植、耐性を誘導し、 in vitroin vivo評価プロトコルを提案します。

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