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Channelrhodopsins'teki İyon Seçiciliğinin Elektrofizyolojik Olarak Belirlenmesi için Tam Hücreli Yama Kelepçesi Kayıtları
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JoVE Journal Neurociência
Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
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Channelrhodopsins'teki İyon Seçiciliğinin Elektrofizyolojik Olarak Belirlenmesi için Tam Hücreli Yama Kelepçesi Kayıtları

16,581 Views

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08:39 min

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May 22, 2017

DOI:

10.3791/55497-v

DOI:
10.3791/55497-v

•

08:39 min
May 22, 2017

•

9 Views
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1Experimental Biophysics, Institute of Biology,Humboldt-Universität zu Berlin

Tags

Whole-cell Patch-clampElectrophysiologyIon SelectivityChannelrhodopsinsHEK CellsTransfectionPhotocurrentBiophysicsIntracellular BufferExtracellular BufferOsmolarityPatch PipetteFire PolishingRecording Chamber

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Grimm, C., Vierock, J., Hegemann, P., Wietek, J. Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins. J. Vis. Exp. (123), e55497, doi:10.3791/55497 (2017).

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