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全细胞贴片记录用于电泳生理测定通道视紫质中的离子选择性
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Neurociência
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JoVE Journal Neurociência
Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
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全细胞贴片记录用于电泳生理测定通道视紫质中的离子选择性

16,581 Views

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08:39 min

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May 22, 2017

DOI:

10.3791/55497-v

DOI:
10.3791/55497-v

•

08:39 min
May 22, 2017

•

9 Views
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1Experimental Biophysics, Institute of Biology,Humboldt-Universität zu Berlin

Tags

Whole-cell Patch-clampElectrophysiologyIon SelectivityChannelrhodopsinsHEK CellsTransfectionPhotocurrentBiophysicsIntracellular BufferExtracellular BufferOsmolarityPatch PipetteFire PolishingRecording Chamber

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Cite this Article

Grimm, C., Vierock, J., Hegemann, P., Wietek, J. Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins. J. Vis. Exp. (123), e55497, doi:10.3791/55497 (2017).

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