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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析

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13:28 min

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May 16, 2017

DOI:

10.3791/55448-v

DOI:
10.3791/55448-v

•

13:28 min
May 16, 2017

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3 Views
, , , , , , , ,
1Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Tags

Single Particle AnalysisElectron Cryo-microscopySPHIREMacromolecular ComplexesHigh-resolution Cryo-EMParticle Box SizeCTF EstimationMotion CorrectionDose-weightedDose-unweightedParticle Picking

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Moriya, T., Saur, M., Stabrin, M., Merino, F., Voicu, H., Huang, Z., Penczek, P. A., Raunser, S., Gatsogiannis, C. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J. Vis. Exp. (123), e55448, doi:10.3791/55448 (2017).

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