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Udarbejdelse af ikke-menneskelig primat Brain Tissue til Pre-indlejring Immunohistokemi og Electron Microscopy

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Udarbejdelse af ikke-menneskelig primat Brain Tissue til Pre-indlejring Immunohistokemi og Electron Microscopy

13,708 Views

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11:55 min

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April 03, 2017

DOI:

10.3791/55397-v

DOI:

10.3791/55397-v

•

11:55 min

April 03, 2017

•

1 Views

Lara Eid², Martin Parent

¹Centre de recherche de l’Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience,Université Laval, ²Centre de recherche du CHU Sainte-Justine

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Non-human Primate Pre-embedding Immunohistochemistry Electron Microscopy Acrolein Fixation Sodium Borohydride Normal Serum Cold Fish Gelatin Primary Antibody Secondary Antibody Avidin-biotin-peroxidase 3 3′-Diaminobenzidine Hydrogen Peroxide Osmium Tetroxide

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