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Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
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Neuroscienze
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JoVE Journal Neuroscienze
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

13,810 Views

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11:55 min

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April 03, 2017

DOI:

10.3791/55397-v

DOI:
10.3791/55397-v

•

11:55 min
April 03, 2017

•

3 Views
,
1Centre de recherche de l’Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience,Université Laval, 2Centre de recherche du CHU Sainte-Justine

Summary

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Here, we provide an easy, low-cost, and time-efficient protocol to chemically fix primate brain tissue with acrolein fixative, allowing for long-term preservation that is compatible with pre-embedding immunohistochemistry for transmission electron microscopy.

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Eid, L., Parent, M. Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy. J. Vis. Exp. (122), e55397, doi:10.3791/55397 (2017).

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