Journal
/
Imunologia e Infecção
/
检测和罕见富集抗原特异性B细胞的表型和功能分析
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Please note that all translations are automatically generated. Click here for the English version.

检测和罕见富集抗原特异性B细胞的表型和功能分析

11,217 Views

•

09:25 min

•

February 16, 2017

DOI:

10.3791/55382-v

DOI:
10.3791/55382-v

•

09:25 min
February 16, 2017

•

7 Views
, , , , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology,Colorado State University, 3Barbara Davis Center for Childhood Diabetes,University of Colorado School of Medicine, 4Department of Biomedical Research,National Jewish Health

Summary

Automatically generated

描述了采用磁性纳米颗粒，以检测和富集的功能和表型分析抗原反应性B细胞中的简单而有效的方法。

Explore More Videos

Rare Antigen-specific B CellsMagnetic NanoparticlesPhenotypic AnalysisFunctional AnalysisImmunologyFlow CytometryDensity Gradient SeparationMononuclear CellsFc Gamma Receptor BlockingFluorescence-conjugated AntibodiesBiotinylated Antigen

Read Article

Cite this Article

Smith, M. J., Packard, T. A., O'Neill, S. K., Hinman, R. M., Rihanek, M., Gottlieb, P. A., Cambier, J. C. Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function. J. Vis. Exp. (120), e55382, doi:10.3791/55382 (2017).

Download .ris file

Copy

Share Video

.

Copy