このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
11,224 Views
•
09:25 min
•
February 16, 2017
DOI:
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology,Colorado State University, 3Barbara Davis Center for Childhood Diabetes,University of Colorado School of Medicine, 4Department of Biomedical Research,National Jewish Health
概要
Automatically generated
A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.
Read Article
Cite this Article
Smith, M. J., Packard, T. A., O'Neill, S. K., Hinman, R. M., Rihanek, M., Gottlieb, P. A., Cambier, J. C. Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function. J. Vis. Exp. (120), e55382, doi:10.3791/55382 (2017).
Copy