/
/
Rationalisé unicellulaire TCR isolement et génération des vecteurs rétroviraux pour In Vitro et In Vivo Expression de TCRs humaines
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Capítulos
- 00:00Título
- 01:42Single cell sort and reverse transcription reaction
- 03:37Multiplex-nested PCR
- 08:56Subcloning TCR alpha and beta chains
- 09:56Verification of TCR expression
- 10:46Conclusion
