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通过诱导瞬时转染可控离子通道表达

JoVE Journal
Genética

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JoVE Journal Genética

Controllable Ion Channel Expression through Inducible Transient Transfection

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通过诱导瞬时转染可控离子通道表达

DOI:

10.3791/55370-v

•

10:00 min

•

February 17, 2017

•

Matan Geron*¹, Adina Hazan*¹, Avi Priel

¹The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine,The Hebrew University of Jerusalem (HUJI)

Capítulos

00:05Título
00:45Transfecting the Plasmid of Interest into Cells
02:27Inducing Gene Expression
03:48Calibrating Timeline of Protein Expression
08:20Results: Response of TRPV1 Activation Increases According to Induction Time as Visualized through Calcium Imaging
09:20Conclusion

Summary

Tadução automática

通过异源表达系统研究离子通道已成为生物医学研究核心技术。在这个手稿，我们提出了一个时间有效的方法通过诱导型启动子的控制下进行瞬时转染来实现紧密控制的离子通道的表达。

Tags

Inducible Transient Transfection Ion Channel Expression TREX 293 Cells TRPV1 EGFP Doxycycline Electrophysiology Calcium Imaging PDL-coated Coverslips/chambers Controlled Protein Expression Heterologous Systems Replicable Results Ion Channel Research Pharmacology

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