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Controllable Ion Channel Expression through Inducible Transient Transfection
Kapitel
- 00:05Titel
- 00:45Transfecting the Plasmid of Interest into Cells
- 02:27Inducing Gene Expression
- 03:48Calibrating Timeline of Protein Expression
- 08:20Results: Response of TRPV1 Activation Increases According to Induction Time as Visualized through Calcium Imaging
- 09:20Conclusion
Studying ion channels through a heterologously expressing system has become a core technique in biomedical research. In this manuscript, we present a time efficient method to achieve tightly controlled ion channel expression by performing transient transfection under the control of an inducible promoter.
