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序贯盐萃取法分析染色质修饰配合物的体染色质结合特性

JoVE Journal
Genética

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JoVE Journal Genética

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

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序贯盐萃取法分析染色质修饰配合物的体染色质结合特性

DOI:

10.3791/55369-v

•

07:41 min

•

October 02, 2017

•

Elizabeth G. Porter, Katelyn E. Connelly, Emily C. Dykhuizen

¹Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University

Capítulos

00:05Título
00:55Basic Sequential Salt Extraction (SSE)
05:10Results: Evaluation of Elution Patterns with Sequential Salt Extraction
07:03Conclusion

Summary

Tadução automática

顺序盐提取染色质结合蛋白是一个有用的工具, 以确定结合性质的大蛋白复合物。该方法可用于评价单个亚基或域在蛋白质复合体与大块染色质的整体亲和性中的作用。

Tags

Keywords: Chromatin Binding Sequential Salt Extraction Chromatin Modifying Complexes Chromatin Immunoprecipitation Epigenetic Regulators Protein Binding Profiles Salt-free Modified Ripa Buffer Sodium Chloride

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