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JoVE Journal Immunology and Infection
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
 

The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies

Article DOI: 10.3791/55347-v • 10:40 min • February 13th, 2017
February 13th, 2017 •
1, 1, 1, 1
1Department of Pathology, UT Southwestern Medical Center

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The ex vivo organ culture allows investigation of biological processes in the context of the intact tissue architecture. Here, we introduce a method of ex vivo culture of the mouse colon, which can be used to study innate immunity and antimicrobial host defense in the intestine.

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Keywords: Ex Vivo Colon Organ Culture Antimicrobial Host Defense Pathogen Associated Molecular Patterns Pattern Recognition Receptors Innate Immune Pathways Antimicrobial Peptides Intestinal Architecture Euthanasia Peritoneum Cecum Rectum PBS DMEM F12 FBS Penicillin Streptomycin Gentamycin Cell Strainer 12-well Cell Culture Plate
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