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用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

JoVE Journal
Ambiente
Author Produced

This content is Free Access.

JoVE Journal Ambiente

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

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用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中

DOI:

10.3791/55310-v

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17:39 min

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July 16, 2017

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Lawrence G. Oates, Harry W. Read, Jessica L. M. Gutknecht, David S. Duncan, Teri B. Balser, Randall D. Jackson

¹Department of Agronomy and Great Lakes Bioenergy Research Center,University of Wisconsin – Madison, ²Department of Soil Science,University of Wisconsin – Madison, ³Department of Soil, Water, and Climate,University of Minnesota, ⁴Faculty of Science and Engineering,Curtin University

Capítulos

00:00Título
00:21Preparations
03:08Day 1 Lipid Extractions
06:02Day 2 Isolation of Lipids
08:02Day 3 Saponification and Methylation
11:10Day 4 Preparation for GC Analysis
12:04GC Analysis
13:40Representative Results
16:37Conclusion

Summary

Tadução automática

该文章描述了一种增加产量，同时平衡从微生物细胞膜提取脂质的努力和准确性，用于表征总脂质和指示性脂质的相对丰度，以确定具有许多样品的研究中的土壤微生物群落结构。

Tags

Keywords: Lipid Extraction Soil Microbes Characterization Throughput Sample Preparation Freeze Drying Grinding Glassware Cleaning Hexane Rinsing Muffle Furnace Teflon Tubes Blanks Check Standard

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