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A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
JoVE Journal
Ambiente
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JoVE Journal Ambiente
A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

DOI:
10.3791/55310-v

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17:39 min

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July 16, 2017

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, , , , ,
1Department of Agronomy and Great Lakes Bioenergy Research Center,University of Wisconsin – Madison, 2Department of Soil Science,University of Wisconsin – Madison, 3Department of Soil, Water, and Climate,University of Minnesota, 4Faculty of Science and Engineering,Curtin University

Capítulos

  • 00:00Título
  • 00:21Preparations
  • 03:08Day 1 Lipid Extractions
  • 06:02Day 2 Isolation of Lipids
  • 08:02Day 3 Saponification and Methylation
  • 11:10Day 4 Preparation for GC Analysis
  • 12:04GC Analysis
  • 13:40Representative Results
  • 16:37Conclusion

Summary

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The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.

Tags

Keywords: Lipid ExtractionSoil MicrobesCharacterizationThroughputSample PreparationFreeze DryingGrindingGlassware CleaningHexane RinsingMuffle FurnaceTeflon TubesBlanksCheck Standard

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