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成年小鼠视网膜电生理研究水平切片的制备
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Neurociência
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JoVE Journal Neurociência
Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
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成年小鼠视网膜电生理研究水平切片的制备

DOI:
10.3791/55173-v

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07:02 min

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January 27, 2017

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1Department of Biology,Friedrich-Alexander-University (FAU) Erlangen-Nürnberg

Capítulos

  • 00:05Título
  • 00:57Dissection of Mouse Retina
  • 03:12Embedding of Retinal Pieces in Agarose
  • 04:14Mounting of Retinal Pieces
  • 04:58Results: Synaptic Activity Recorded from a Horizontal Cell Body
  • 06:24Conclusion

Summary

Tadução automática

Tadução automática

我们开发了成年哺乳动物视网膜的水平切片准备具有平行切片于视网膜表面的平面。非径向导向的视网膜神经元树突领域并没有截断，使信号处理与膜片钳和成像技术研究。

Tags

Horizontal SlicesAdult Mouse RetinaElectrophysiological StudiesRetinal NeuronsRibbon SynapsesVisual Information ProcessingHorizontal CellsAmacrine CellsEyeball DissectionCornea RemovalVitreous Body RemovalRetina Isolation

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