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Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
JoVE Journal
神経科学
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JoVE Journal 神経科学
Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

DOI:

07:02 min

January 27, 2017

,

  • 00:05標題
  • 00:57Dissection of Mouse Retina
  • 03:12Embedding of Retinal Pieces in Agarose
  • 04:14Mounting of Retinal Pieces
  • 04:58Results: Synaptic Activity Recorded from a Horizontal Cell Body
  • 06:24Conclusion

概要

自動翻訳

We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques.

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