/
/
Methode om te visualiseren en te analyseren Membrane interagerende eiwitten door Transmission Electron Microscopy
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Methode om te visualiseren en te analyseren Membrane interagerende eiwitten door Transmission Electron Microscopy
12,935 Views
•
10:49 min
•
March 05, 2017
DOI:
Summary
Automatically generated
Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.
Read Article
Cite this Article
B. Kumar, R., Zhu, L., Hebert, H., Jegerschöld, C. Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy. J. Vis. Exp. (121), e55148, doi:10.3791/55148 (2017).
Copy