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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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Biochemie
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JoVE Journal Biochemie
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

DOI:
10.3791/55148-v

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10:49 min

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March 05, 2017

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, , ,
1Department of Biosciences and Nutrition,Karolinska Institutet, 2School of Technology and Health,KTH Royal Institute of Technology

Kapitel

  • 00:05Titel
  • 01:08Preparation, Reconstitution, and Analysis of Empty Nanodiscs
  • 05:11Preparation and Analysis of the Nanodisc-Monotopic Protein Complex
  • 07:56Results: Monotopic Membrane Protein Binding on the Surfaces of Empty Nanodiscs (NDs)
  • 09:26Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.

Tags

Membrane ProteinsTransmission Electron MicroscopyNanodiscMSP1E3D1POPCSodium CholateProtein-membrane InteractionDrug DevelopmentStructural Analysis

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