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Open Source Content Haute Analyse Utilisant automatisé Fluorescence Lifetime Imaging Microscopy
JoVE Journal
Biologia
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JoVE Journal Biologia
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Open Source Content Haute Analyse Utilisant automatisé Fluorescence Lifetime Imaging Microscopy

11,680 Views

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09:30 min

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January 18, 2017

DOI:

10.3791/55119-v

DOI:
10.3791/55119-v

•

09:30 min
January 18, 2017

•

3 Views
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1Photonics Group, Department of Physics,Imperial College London, 2Institute for Chemical Biology, Department of Chemistry,Imperial College London, 3MRC Clinical Sciences Centre,Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry,Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development,Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology,Imperial College London

Summary

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Nous présentons une open source haute analyse de contenu (HCA) en utilisant l'instrument automatisé d'imagerie de fluorescence à vie (FLIM) pour analyser les interactions entre protéines en utilisant Förster transfert d'énergie par résonance (FRET) Affichages sur la base. L' acquisition de données pour cet instrument openFLIM-HCA est contrôlé par un logiciel écrit en μManager et l' analyse des données est effectuée en FLIMfit.

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Görlitz, F., Kelly, D. J., Warren, S. C., Alibhai, D., West, L., Kumar, S., Alexandrov, Y., Munro, I., Garcia, E., McGinty, J., Talbot, C., Serwa, R. A., Thinon, E., da Paola, V., Murray, E. J., Stuhmeier, F., Neil, M. A. A., Tate, E. W., Dunsby, C., French, P. M. W. Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (119), e55119, doi:10.3791/55119 (2017).

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