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Produktion, Kristallisation und Strukturbestimmung<em> C. difficile</em> PPEP-1 über Microseeding und Zink-SAD
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Produktion, Kristallisation und Strukturbestimmung<em> C. difficile</em> PPEP-1 über Microseeding und Zink-SAD
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13:34 min
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December 30, 2016
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Summary
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Proline-proline endopeptidase-1 (PPEP-1) is a secreted metalloprotease and promising drug-target from the human pathogen Clostridium difficile. Here we describe all methods necessary for the production and structure determination of this protein.
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Pichlo, C., Montada, A. A., Schacherl, M., Baumann, U. Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD. J. Vis. Exp. (118), e55022, doi:10.3791/55022 (2016).
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