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تحديد موثوق المعيشة الدوبامين الخلايا العصبية في الدماغ المتوسط ​​الثقافات عن طريق RNA تسلسل وTH يحركها المروج EGFP التعبير
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Neurociência
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JoVE Journal Neurociência
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression
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تحديد موثوق المعيشة الدوبامين الخلايا العصبية في الدماغ المتوسط ​​الثقافات عن طريق RNA تسلسل وTH يحركها المروج EGFP التعبير

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10:54 min

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February 10, 2017

DOI:

10.3791/54981-v

DOI:
10.3791/54981-v

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10:54 min
February 10, 2017

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1Division of Biology and Biological Engineering,California Institute of Technology (Caltech)

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RNA SequencingTyrosine HydroxylaseEGFPDopaminergic NeuronsMidbrain CulturesParkinson’s DiseaseDrug AddictionVentral MesencephalonVentral Tegmental AreaSubstantia NigraDissection

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Henley, B. M., Cohen, B. N., Kim, C. H., Gold, H. D., Srinivasan, R., McKinney, S., Deshpande, P., Lester, H. A. Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression. J. Vis. Exp. (120), e54981, doi:10.3791/54981 (2017).

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