Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

Beverley M. Henley; Bruce N. Cohen; Charlene H. Kim; Heather D. Gold; Rahul Srinivasan; Sheri McKinney; Purnima Deshpande; Henry A. Lester

doi:10.3791/54981

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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

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神経科学

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JoVE Journal 神経科学

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

9,207 Views

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10:54 min

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February 10, 2017

DOI:

10.3791/54981-v

DOI:

10.3791/54981-v

•

10:54 min

February 10, 2017

•

1 Views

Beverley M. Henley¹, Bruce N. Cohen¹, Charlene H. Kim¹, Heather D. Gold¹, Rahul Srinivasan¹, Sheri McKinney¹, Purnima Deshpande¹, Henry A. Lester¹

¹Division of Biology and Biological Engineering,California Institute of Technology (Caltech)

概要

In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using RNA-seq.

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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

概要

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