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Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
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JoVE Journal Genética
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

DOI:
10.3791/54969-v

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08:48 min

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December 21, 2016

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, , , , ,
1Department of Occupational and Environmental Health,University of Arkansas for Medical Sciences, 2Department of Environmental Health Sciences,University of Alabama at Birmingham, 3Division of Radiation Health,University of Arkansas for Medical Sciences

Capítulos

  • 00:05Título
  • 00:35Particle Exposure
  • 02:12Cell Treatment with Particulate Matter
  • 03:21Cell Harvest, Hypotonic Treatment, and Fixation
  • 05:35Slide Cleaning and Metaphase Spread Preparation
  • 07:12Results: Chromosomal Aberrations from Particulate Matter on Metaphase Spreads
  • 08:08Conclusion

Summary

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This protocol describes techniques for the quantification and characterization of chromosomal aberrations in vitro in RAW264.7 mouse macrophages after treatment with ambient air particulate matter.

Tags

Chromosomal AberrationsParticulate MatterIn VitroAir PollutionCell CultureGenotoxicityMetaphase ArrestTrypsinCell SuspensionCell DensityCell IncubationPBSColcemid

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