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Inducibile LAP-tagged linee cellulari stabili per indagare la funzione delle proteine, Spatiotemporal Localizzazione e reti di interazione proteina

JoVE Journal
Biologia

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JoVE Journal Biologia

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

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Inducibile LAP-tagged linee cellulari stabili per indagare la funzione delle proteine, Spatiotemporal Localizzazione e reti di interazione proteina

9,598 Views

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11:04 min

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December 24, 2016

DOI:

10.3791/54870-v

DOI:

10.3791/54870-v

•

11:04 min

December 24, 2016

•

1 Views

Michelle Bradley, Ivan Ramirez, Keith Cheung, Ankur A. Gholkar, Jorge Z. Torres^2,3

¹Department of Chemistry and Biochemistry,University of California, Los Angeles, ²Molecular Biology Institute,University of California, Los Angeles, ³Jonsson Comprehensive Cancer Center,University of California, Los Angeles

Tags

Inducible LAP-tagged Stable Cell Lines Protein Function Spatiotemporal Localization Protein Interaction Networks HEK293 Cells Hygromycin Selection Tandem Affinity Purification (TAP)Tet/Dox Induction Anti-GFP Antibody Protein A Beads

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