Journal
/
Biologie
/
Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

9,598 Views

•

11:04 min

•

December 24, 2016

DOI:

10.3791/54870-v

DOI:
10.3791/54870-v

•

11:04 min
December 24, 2016

•

1 Views
, , , ,
1Department of Chemistry and Biochemistry,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles, 3Jonsson Comprehensive Cancer Center,University of California, Los Angeles

Tags

Inducible LAP-taggedStable Cell LinesProtein FunctionSpatiotemporal LocalizationProtein Interaction NetworksHEK293 CellsHygromycin SelectionTandem Affinity Purification (TAP)Tet/Dox InductionAnti-GFP AntibodyProtein A Beads

Read Article

Cite this Article

Bradley, M., Ramirez, I., Cheung, K., Gholkar, A. A., Torres, J. Z. Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks. J. Vis. Exp. (118), e54870, doi:10.3791/54870 (2016).

Download .ris file

Copy

Share Video

.

Copy