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粒子结合Bet v 1的变应原PM10的流式细胞分析
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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粒子结合Bet v 1的变应原PM10的流式细胞分析

7,207 Views

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10:42 min

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November 19, 2016

DOI:

10.3791/54721-v

DOI:
10.3791/54721-v

•

10:42 min
November 19, 2016

•

5 Views
, , ,
1Environmental Medicine and Health Effects Assessment,Federal Environment Agency

Summary

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在这里，我们提出了一个协议，通过流式细胞仪量化过敏原吸附颗粒。周围的颗粒物质的颗粒可充当吸附变应原的载体。我们在这里显示，流式细胞仪中，一种方法被广泛用于表征悬浮固体>在直径为0.5微米，可以用来测量这些过敏原载粒子。

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Cite this Article

Süring, K., Bach, S., Höflich, C., Straff, W. Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10. J. Vis. Exp. (117), e54721, doi:10.3791/54721 (2016).

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