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Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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November 19, 2016
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Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.
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Süring, K., Bach, S., Höflich, C., Straff, W. Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10. J. Vis. Exp. (117), e54721, doi:10.3791/54721 (2016).
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