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Tools, um die Rolle der Architektur-Protein HMGB1 zur Untersuchung bei der Verarbeitung von Helix verzerrenden, ortsspezifische DNA-Quervernetzungen zwischen
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JoVE Journal Genética
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
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Tools, um die Rolle der Architektur-Protein HMGB1 zur Untersuchung bei der Verarbeitung von Helix verzerrenden, ortsspezifische DNA-Quervernetzungen zwischen

8,137 Views

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12:19 min

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November 10, 2016

DOI:

10.3791/54678-v

DOI:
10.3791/54678-v

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12:19 min
November 10, 2016

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1 Views
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1Division of Pharmacology and Toxicology, College of Pharmacy,The University of Texas at Austin

Tags

HMGB1Architectural ProteinDNA Interstrand CrosslinksChromatin ImmunoprecipitationDNA SupercoilingSite-specific DNA DamageCancer TherapyTFO-directed ICLDNA RepairMammalian CellsTransfectionFormaldehyde CrosslinkingGlycine QuenchingCell CollectionCentrifugation

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Mukherjee, A., Vasquez, K. M. Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks. J. Vis. Exp. (117), e54678, doi:10.3791/54678 (2016).

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