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Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
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Genetica
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JoVE Journal Genetica
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

8,151 Views

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12:19 min

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November 10, 2016

DOI:

10.3791/54678-v

DOI:
10.3791/54678-v

•

12:19 min
November 10, 2016

•

7 Views
,
1Division of Pharmacology and Toxicology, College of Pharmacy,The University of Texas at Austin

Summary

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Targeted DNA damage can be achieved by tethering a DNA damaging agent to a triplex-forming oligonucleotide (TFO). Using modified TFOs, DNA damage-specific protein association, and DNA topology modification can be studied in human cells by the utilization of modified chromatin immunoprecipitation assays and DNA supercoiling assays described herein.

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Mukherjee, A., Vasquez, K. M. Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks. J. Vis. Exp. (117), e54678, doi:10.3791/54678 (2016).

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