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Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
 

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Article DOI: 10.3791/54631-v • 08:36 min • October 9th, 2017
October 9th, 2017 •
1,2, 2, 3,4, 5, 1,3, 6,7,8
1Vegetable Crop Research Unit, USDA-ARS, 2Department of Entomology, University of Wisconsin-Madison, 3Department of Horticulture, University of Wisconsin-Madison, 4Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, 5Department of Bacteriology, University of Wisconsin-Madison, 6Laboratory of Genetics, Genome Center of Wisconsin, 7DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, 8J.F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison

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Resumo
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Microbial consortia within bumble bee hives enrich and preserve pollen for bee larvae. Using next generation sequencing, along with laboratory and field-based experiments, this manuscript describes protocols used to test the hypothesis that fungicide residues alter the pollen microbiome, and colony demographics, ultimately leading to colony loss.

Tags

Empirical Techniques Metagenomic Techniques Computational Techniques Fungicides Bee Health Colony Declines Bumblebees Fungicide Residue Pollen Larval Bumblebees Bee Microbes Symbioses Microbial Communities Bee Demographics Honeybees Pollinators Flowering Crops Mesh Cages Oats Potted Flowering Plants
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