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Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
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Biologia
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JoVE Journal Biologia
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

DOI:
10.3791/54630-v

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10:35 min

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October 19, 2016

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, , , ,
1Program in Translational Biomechanics, Institute of Translational Medicine and Therapeutics,University of Pennsylvania, 2Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, 3Department of Physiology,University of Pennsylvania

Capítulos

  • 00:05Título
  • 00:55Preparing the Mouse and Isolation of the Aorta
  • 02:06Preparing Tissue Samples for the AFM Measurements
  • 02:46Loading the AFM Probe and Aligning the Laser
  • 04:16Calibrating the Deflection Sensitivity and Spring Constant of the AFM Probe
  • 06:28Measuring the Elastic Modulus on Mouse Arteries Ex Vivo
  • 07:50Data Analysis
  • 08:47Results: Determining Aortic Stiffness
  • 09:29Conclusion

Summary

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We present detailed protocols for isolation of aortas from mouse and measurement of their elastic modulus using atomic force microscopy.

Tags

Keywords: AortaAtomic Force MicroscopyEx VivoElastic ModulusVascular BiomechanicsCardiovascular DiseaseAgingSoft Biological SamplesDissectionPBSCyanoacrylate AdhesiveAFM ProbeContact Mode

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