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מדידת טיטר נוגדנים עיכוב Neuraminidase שפעת ידי enzyme-linked Assay לקטינים
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

מדידת טיטר נוגדנים עיכוב Neuraminidase שפעת ידי enzyme-linked Assay לקטינים

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08:11 min

September 06, 2016

DOI:

08:11 min
September 06, 2016

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Summary

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We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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