Journal
/
Bioquímica
/
준비 및 직렬 펨토초 결정학에 대한 지질 큐빅 단계에서 단백질 미세 배달
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Please note that all translations are automatically generated. Click here for the English version.

준비 및 직렬 펨토초 결정학에 대한 지질 큐빅 단계에서 단백질 미세 배달

11,098 Views

•

09:09 min

•

September 20, 2016

DOI:

10.3791/54463-v

DOI:
10.3791/54463-v

•

09:09 min
September 20, 2016

•

9 Views
, ,
1The Bridge Institute,University of Southern California, 2Department of Chemistry,University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

Summary

Automatically generated

We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

Explore More Videos

Protein MicrocrystalsLipidic Cubic PhaseSerial Femtosecond CrystallographyMembrane Protein StructureProtein ReconstitutionSample PreparationSyringe HandlingPrecipitant SolutionProtein-laden LCPTeflon SpherulesSample Sealing

Read Article

Cite this Article

Ishchenko, A., Cherezov, V., Liu, W. Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. J. Vis. Exp. (115), e54463, doi:10.3791/54463 (2016).

Download .ris file

Copy

Share Video

.

Copy