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Preparation of Homogeneous MALDI Samples for Quantitative Applications
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JoVE Journal Bioquímica
Preparation of Homogeneous MALDI Samples for Quantitative Applications

Preparation of Homogeneous MALDI Samples for Quantitative Applications

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08:01 min

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October 28, 2016

DOI:

10.3791/54409-v

DOI:
10.3791/54409-v

•

08:01 min
October 28, 2016

•

25 Views
, , , , ,
1Genomic Research Center,Academia Sinica, 2Department of Chemistry,National Taiwan University, 3Department of Chemical Engineering and Biotechnology,National Taipei University of Technology

Summary

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A protocol for reducing spatial heterogeneities of ion signals in MALDI mass spectrometry by regulating substrate temperature during sample drying processes is demonstrated.

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MALDISample PreparationHomogeneous SamplesQuantitative ApplicationsDried-droplet MethodSpatial HeterogeneityDrying EnvironmentHydrodynamic FlowsTHAP Metric SystemAlpha-Cyano-4-hydroxycinnamic AcidDrying ChamberNitrogen FlowRelative HumidityTemperature ControlSample DepositionAnalyteCrystallization

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Ou, Y., Tsao, C., Lai, Y., Lee, H., Chang, H., Wang, Y. Preparation of Homogeneous MALDI Samples for Quantitative Applications. J. Vis. Exp. (116), e54409, doi:10.3791/54409 (2016).

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