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实验教学中乳糖酶活性的测定
JoVE Journal
Biologia
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JoVE Journal Biologia
Measuring Lactase Enzymatic Activity in the Teaching Lab
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实验教学中乳糖酶活性的测定

DOI:
10.3791/54377-v

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04:41 min

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August 06, 2018

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, , , , ,
1School of Pharmacy,University of Reading, 2Department of Molecular Neuroscience,UCL Institute of Neurology, 3School of Biological Sciences, Royal Hollway,University of London

Capítulos

  • 00:04Título
  • 00:23Enzyme Extraction
  • 01:20Observing the Colorimetric Reaction
  • 01:56Measuring Enzyme Activity
  • 03:05Effect of Temperature on Enzyme Activity
  • 03:53Results: Analysis of Enzymatic Activity Based on Temperature
  • 04:25Conclusion

Summary

Tadução automática

Tadução automática

乳糖酶活性对双糖乳糖的分解加工至关重要。在这里, 在膳食补充剂中发现的乳糖酶活性是用比色法测定的。这为学生提供了一个实验平台, 以了解乳糖酶的活性和酵素动力学。

Tags

LactaseEnzyme ActivityTeaching LabONPGPBSSpectrophotometryTemperatureEnzyme Kinetics

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