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الهندسة الوراثية من الخميرة غير تقليدية للالمتجددة الوقود الحيوي وإنتاج البيوكيميائية
JoVE Journal
Genética
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JoVE Journal Genética
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
DOI:

10:10 min

September 20, 2016

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Capítulos

  • 00:05Título
  • 01:18Construction of the Disruption Cassette for Deleting KU70
  • 03:08Generation of the Y. lipolytica KU70 Deletion Strain
  • 05:45Cre Recombinase-mediated Marker Rescue
  • 07:59Results: Deletion of KU70 Enhances Gene Deletion Efficiency
  • 09:27Conclusion

Summary

Tadução automática

We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.

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