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Biologia do Desenvolvimento
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In vitro og in vivo modeller til at studere hornhindeendothel-mesenkymale Transition
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

In vitro og in vivo modeller til at studere hornhindeendothel-mesenkymale Transition

DOI:
10.3791/54329-v

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09:05 min

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August 20, 2016

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, , , , , ,
1Department of Ophthalmology,Far Eastern Memorial Hospital, 2Institute of Clinical Medicine,National Taiwan University, 3Department of Ophthalmology,National Taiwan University Hospital, 4College of Medicine,National Taiwan University

Capítulos

  • 00:05Título
  • 01:06Primary Preparation and Immunostaining of Bovine Corneal Endothelial Cells
  • 04:24Rat Corneal Endothelium Cryoinjury Model and Intracameral Injection
  • 06:46Results: Immunostaining of In Vitro Cultured Bovine CECs with Marimastat Rat Corneal Buttons After Cryoinjury
  • 08:27Conclusion

Summary

Tadução automática

Tadução automática

En primær kultur af bovine hornhindeendothelceller blev anvendt til undersøgelse af mekanismen af ​​hornhindeendothel-mesenkymal overgang. Endvidere blev en rotte hornhindeendothel kryobeskadigelse model, der anvendes til at demonstrere hornhindeendothel-mesenchymal overgang in vivo.

Tags

Keywords: Corneal Endothelial-mesenchymal TransitionIn VitroIn VivoCorneal EndotheliumRegenerationBovine EyesDescemet’s MembraneCorneal Endothelial CellsSHEMTrypsinHemacytometerMaramastatParaformaldehydeTriton X-100BSAABCSnailSlug

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