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Biologia do Desenvolvimento

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在体外和体内模型来研究角膜内皮-间充质转换

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

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在体外和体内模型来研究角膜内皮-间充质转换

DOI:

10.3791/54329-v

•

09:05 min

•

August 20, 2016

•

Wei-Ting Ho², Chien-Chia Su, Jung-Shen Chang, Shu-Wen Chang, Fung-Rong Hu, Tzuu-Shuh Jou⁴, I-Jong Wang⁴

¹Department of Ophthalmology,Far Eastern Memorial Hospital, ²Institute of Clinical Medicine,National Taiwan University, ³Department of Ophthalmology,National Taiwan University Hospital, ⁴College of Medicine,National Taiwan University

Capítulos

00:05Título
01:06Primary Preparation and Immunostaining of Bovine Corneal Endothelial Cells
04:24Rat Corneal Endothelium Cryoinjury Model and Intracameral Injection
06:46Results: Immunostaining of In Vitro Cultured Bovine CECs with Marimastat Rat Corneal Buttons After Cryoinjury
08:27Conclusion

Summary

Tadução automática

牛角膜内皮细胞的原代培养物用于研究角膜内皮 - 间充质转换的机制。此外，大鼠角膜内皮冷冻损伤模型被用来证明在体内角膜内皮-间充质转换。

Tags

Keywords: Corneal Endothelial-mesenchymal Transition In Vitro In Vivo Corneal Endothelium Regeneration Bovine Eyes Descemet’s Membrane Corneal Endothelial Cells SHEM Trypsin Hemacytometer Maramastat Paraformaldehyde Triton X-100 BSA ABC Snail Slug

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