Journal
/
Biologia do Desenvolvimento
/
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

DOI:
10.3791/54329-v

•

09:05 min

•

August 20, 2016

•

, , , , , ,
1Department of Ophthalmology,Far Eastern Memorial Hospital, 2Institute of Clinical Medicine,National Taiwan University, 3Department of Ophthalmology,National Taiwan University Hospital, 4College of Medicine,National Taiwan University

Capítulos

  • 00:05Título
  • 01:06Primary Preparation and Immunostaining of Bovine Corneal Endothelial Cells
  • 04:24Rat Corneal Endothelium Cryoinjury Model and Intracameral Injection
  • 06:46Results: Immunostaining of In Vitro Cultured Bovine CECs with Marimastat Rat Corneal Buttons After Cryoinjury
  • 08:27Conclusion

Summary

Tadução automática

Tadução automática

A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.

Tags

Keywords: Corneal Endothelial-mesenchymal TransitionIn VitroIn VivoCorneal EndotheliumRegenerationBovine EyesDescemet’s MembraneCorneal Endothelial CellsSHEMTrypsinHemacytometerMaramastatParaformaldehydeTriton X-100BSAABCSnailSlug

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54329?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/pt/54329?article_type=v

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
check_url/pt/54329?article_type=v

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
check_url/pt/54329?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/pt/54329?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/pt/54329?article_type=v

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
check_url/pt/54329?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/pt/54329?article_type=v

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue
check_url/pt/54329?article_type=v

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues
check_url/pt/54329?article_type=v

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Read Article